The shooting happened around 10 p.m. Sunday in a Sarasota neighborhood.
The officers were on bicycle patrol when they encountered the man, who pulled the gun on them. Police say the officers ordered the man to put the weapon down, but he refused and was shot.
Emergency crews tried to revive the man but he died.
The two officers have been placed on administrative leave pending the outcome on an ongoing investigation.
The names of the suspect and the officers have not been released.
No further details were immediately available.
Source: Associated Press